Photo Not Available Cindy Huang




Phloem Long-distance trafficking of GIBBERLLIC ACID INSESITIVE RNA in Arabidopsis

 

Observations have shown that RNA is transported as an information molecule through the phloem and have suggested that RNA can be a regulatory factor to control plant development1,2,3.  Recently, one experiment was done to test long-distance RNA movement using GIBBERELLIC ACID INSETENTITIVE (GAI) RNA, which is grafted-transmissible in plants4.  gai RNA was found to be transported over long distance within phloem; grafted tomato plants display a relatively dark green phenotype compared to wild type phenotype.  The same experiment was performed on Arabidopsis, gai RNA was detected from scions; however, no severe mutant phenotype was observed.  At least two possibilities for this result exist; one possibility is that gai RNA expression is blocked by other molecules, and secondly,  phenotypic alteration in scions is present at levels that could not be detected.  Recent studies have shown that gai RNA is a positive regulator that can induce GA4 and GA5 gene expression.  In this experiment, we hypothesize that gai RNA has the capacity to move over long distance in Arabidopsis and induce GA4 and GA5 gene expression5,6.  Observing increased levels of GA4 and GA5 gene expression in the wild-type scions that have been grafted onto gai stock plants would support the hypothesis that gai RNA is translocated through the phloem from stock to scion.  

Reference

  1. Haywood, V., Kragler, F., and Lucas, W.J. (2002). Plasmodesmata: Pathways for RNA & ribonucleoprotein trafficking. Plant Cell 14, S303-S325
  2. Lucas, W.J., Yoo, B.-C, and Kragerler, F. (2001) RNA as a long-distance information macromolecule in plants. Nature Rev. Mol. Cell Bio. 2, 849-857