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Cindy Huang |
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Phloem Long-distance trafficking of GIBBERLLIC
ACID INSESITIVE RNA in Arabidopsis
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Observations have shown that RNA
is transported as an information molecule through the phloem and
have suggested that RNA can be a regulatory factor to control plant
development1,2,3. Recently, one experiment was done to test
long-distance RNA movement using GIBBERELLIC ACID INSETENTITIVE (GAI)
RNA, which is grafted-transmissible in plants4. gai RNA
was found to be transported over long distance within phloem; grafted
tomato plants display a relatively dark green phenotype compared
to wild type phenotype. The same experiment was performed
on Arabidopsis, gai RNA was detected from scions; however,
no severe mutant phenotype was observed. At least two possibilities
for this result exist; one possibility is that gai RNA
expression is blocked by other molecules, and secondly, phenotypic
alteration in scions is present at levels that could not be detected. Recent
studies have shown that gai RNA is a positive regulator
that can induce GA4 and GA5 gene expression. In
this experiment, we hypothesize that gai RNA has the capacity
to move over long distance in Arabidopsis and induce GA4 and GA5 gene
expression5,6. Observing increased levels of GA4 and GA5 gene
expression in the wild-type scions that have been grafted onto gai stock
plants would support the hypothesis that gai RNA is translocated
through the phloem from stock to scion. |
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Reference
- Haywood, V., Kragler, F., and Lucas, W.J. (2002).
Plasmodesmata: Pathways for RNA & ribonucleoprotein trafficking.
Plant Cell 14, S303-S325
- Lucas, W.J., Yoo, B.-C, and Kragerler, F. (2001)
RNA as a long-distance information macromolecule in plants.
Nature Rev. Mol. Cell Bio. 2, 849-857
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